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Osteonectin / SPARC Antibody (clone ON1-1)

LS-C83590
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetSPARC
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    Osteonectin / SPARC Antibody (clone ON1-1)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Reducing and non-reducing conditions.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IHC, IHC-Fr (1 - 10 µg/ml), IHC-P (1 - 10 µg/ml), WB (1 - 10 µg/ml) Western Blot: Reducing and non-reducing conditions.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    ON1-1
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6678
  • Target name
    SPARC
  • Target description
    secreted protein acidic and cysteine rich
  • Target synonyms
    BM-40, OI17, ON, ONT, SPARC, basement-membrane protein 40, secreted protein, acidic, cysteine-rich (osteonectin)
  • Host
    Mouse
  • Isotype
    IgG1
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A375 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa.
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