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SPARC Antibody (YA1202, Rabbit)

HY-P81457
MedChem Express
TargetSPARC
Product group Antibodies
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Overview

  • Supplier
    MedChem Express
  • Product Name
    SPARC Antibody (YA1202, Rabbit)
  • Delivery Days Customer
    5
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Gene ID6678
  • Target name
    SPARC
  • Target description
    secreted protein acidic and cysteine rich
  • Target synonyms
    BM-40, OI17, ON, ONT, SPARC, basement-membrane protein 40, secreted protein, acidic, cysteine-rich (osteonectin)
  • Host
    Rabbit
  • Isotype
    IgG
  • Scientific Description
    SPARC Antibody (YA1202) is a rabbit-derived non-conjugated IgG antibody (Clone NO.: YA1202), targeting SPARC. SPARC Antibody (YA1202) can be used for IHC-P experiment in human background.
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of SPARC using anti-SPARC antibody (A00862-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human HUVEC whole cell lysates, Lane 2: human placenta tissue lysates, Lane 3: human A375 whole cell lysates, Lane 4: human U20S whole cell lysates, Lane 5: human U87 whole cell lysates, Lane 6: rat testis tissue lysates, Lane 7: rat kidney tissue lysates, Lane 8: mouse testis tissue lysates, Lane 9: mouse kidney tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SPARC antigen affinity purified polyclonal antibody (Catalog # A00862-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SPARC at approximately 35 kDa. The expected band size for SPARC is at 35 kDa.
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