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ProductsBack/OTUB1 antibody [AT17E10]
WB analysis of various samples using GTX57636 OTUB1 antibody. Lane 1 : A549 whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : HepG2 whole cell lysate Lane 4 : 293T whole cell lysate Loading : 40 μg Dilution : 1:5000
WB analysis of various samples using GTX57636 OTUB1 antibody. Lane 1 : A549 whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : HepG2 whole cell lysate Lane 4 : 293T whole cell lysate Loading : 40 μg Dilution : 1:5000
WB analysis of various samples using GTX57636 OTUB1 antibody. Lane 1 : A549 whole cell lysate Lane 2 : HeLa whole cell lysate Lane 3 : HepG2 whole cell lysate Lane 4 : 293T whole cell lysate Loading : 40 μg Dilution : 1:5000

OTUB1 antibody [AT17E10]

GTX57636
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetOTUB1
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Overview

  • Supplier
    GeneTex
  • Product Name
    OTUB1 antibody [AT17E10]
  • Delivery Days Customer
    9
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    AT17E10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID55611
  • Target name
    OTUB1
  • Target description
    OTU deubiquitinase, ubiquitin aldehyde binding 1
  • Target synonyms
    HSPC263, OTB1, OTU1, ubiquitin thioesterase OTUB1, OTU domain, ubiquitin aldehyde binding 1, OTU domain-containing ubiquitin aldehyde-binding protein 1, OTU-domain Ubal-binding 1, deubiquitinating enzyme OTUB1, otubain-1, ubiquitin-specific protease otubain 1, ubiquitin-specific-processing protease OTUB1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDQ96FW1
  • Protein Name
    Ubiquitin thioesterase OTUB1
  • Scientific Description
    The product of this gene is a member of the OTU (ovarian tumor) superfamily of predicted cysteine proteases. The encoded protein is a highly specific ubiquitin iso-peptidase, and cleaves ubiquitin from branched poly-ubiquitin chains but not from ubiquitinated substrates. It interacts with another ubiquitin protease and an E3 ubiquitin ligase that inhibits cytokine gene transcription in the immune system. It is proposed to function in specific ubiquitin-dependent pathways, possibly by providing an editing function for polyubiquitin chain growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of OTUB1 using anti-OTUB1 antibody (A04361-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: rat brain tissue lysates, Lane 4: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUB1 antigen affinity purified polyclonal antibody (Catalog # A04361-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUB1 at approximately 36 kDa. The expected band size for OTUB1 is at 31 kDa.
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