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Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: OTUD5 antibody at 6.75ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 59, 60, 40 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: OTUD5 antibody at 6.75ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 59, 60, 40 kDa Observed band size: 59 kDa
Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: OTUD5 antibody at 6.75ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 61, 59, 60, 40 kDa Observed band size: 59 kDa

OTUD5 Antibody

CSB-PA850298LA01HU
Cusabio
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman
TargetOTUD5
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Overview

  • Supplier
    Cusabio
  • Product Name
    OTUD5 Antibody
  • Delivery Days Customer
    20
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID55593
  • Target name
    OTUD5
  • Target description
    OTU deubiquitinase 5
  • Target synonyms
    DUBA, MCAND, OTU domain-containing protein 5, OTU domain containing 5, deubiquinating enzyme A, deubiquitinase A, deubiquitinating enzyme A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ96G74
  • Protein Name
    OTU domain-containing protein 5
  • Scientific Description
    Deubiquitinating enzyme that functions as negative regulator of the innate immune system. Acts via TRAF3 deubiquitination and subsequent suppression of type I interferon (IFN) production. Has peptidase activity towards Lys-48- and Lys-63-linked polyubiquitin chains. Can also cleave Lys-11-linked ubiquitin chains (in vitro).
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of OTUD5 using anti-OTUD5 antibody (A09744-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human 293T whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Jurkat whole cell lysates, Lane 4: human K562 whole cell lysates, Lane 5: rat liver tissue lysates, Lane 6: rat C6 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-OTUD5 antigen affinity purified polyclonal antibody (Catalog # A09744-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for OTUD5 at approximately 75 kDa. The expected band size for OTUD5 is at 61 kDa.
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