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ProductsBack/OX40 / CD134 / TNFRSF4 (Immuno-Oncology Target) Antibody

OX40 / CD134 / TNFRSF4 (Immuno-Oncology Target) Antibody

ORB751271
Biorbyt
ApplicationsELISA, ImmunoHistoChemistry
Product group Antibodies
TargetTNFRSF4
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Overview

  • Supplier
    Biorbyt
  • Product Name
    OX40 / CD134 / TNFRSF4 (Immuno-Oncology Target) Antibody
  • Delivery Days Customer
    10
  • Application Supplier Note
    Positive Control: MOLT4, HuT78 cells or human peripheral blood leukocytes (hPBL).. ELISA (For coating, order antibody without BSA);,Immunohistochemistry (Formalin-fixed) (1-2ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined. ELISA, IHC
  • Applications
    ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    OX40/3427
  • Concentration
    200 ug/ml
  • Conjugate
    Unconjugated
  • Gene ID7293
  • Target name
    TNFRSF4
  • Target description
    TNF receptor superfamily member 4
  • Target synonyms
    ACT35; ACT35 antigen; ATC35 antigen; CD134; CD134 antigen; IMD16; lymphoid activation antigene ACT35; OX40; OX40 antigen; OX40 cell surface antigen; OX40 homologue; OX40L receptor; TAX transcriptionally-activated glycoprotein 1 receptor; tax-transcriptionally activated glycoprotein 1 receptor; tumor necrosis factor receptor superfamily member 4; TXGP1L
  • Host
    Mouse
  • Isotype
    IgG2c
  • Protein IDP43489
  • Protein Name
    Tumor necrosis factor receptor superfamily member 4
  • Scientific Description
    Mouse monoclonal antibody to TNFRSF4
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of CD134 using anti-CD134 antibody (PB9898). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: SW620 whole cell lysates, Lane 2: 22RV1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD134 antigen affinity purified polyclonal antibody (Catalog # PB9898) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD134 at approximately 50KD. The expected band size for CD134 is at 50KD.
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