More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/p27 Kip1 antibody

p27 Kip1 antibody

GTX73793
GeneTex
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetCDKN1B
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    p27 Kip1 antibody
  • Delivery Days Customer
    9
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID1027
  • Target name
    CDKN1B
  • Target description
    cyclin dependent kinase inhibitor 1B
  • Target synonyms
    CDKN4; cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); KIP1; MEN1B; MEN4; P27KIP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP46527
  • Protein Name
    Cyclin-dependent kinase inhibitor 1B
  • Scientific Description
    This gene encodes a cyclin-dependent kinase inhibitor, which shares a limited similarity with CDK inhibitor CDKN1A/p21. The encoded protein binds to and prevents the activation of cyclin E-CDK2 or cyclin D-CDK4 complexes, and thus controls the cell cycle progression at G1. The degradation of this protein, which is triggered by its CDK dependent phosphorylation and subsequent ubiquitination by SCF complexes, is required for the cellular transition from quiescence to the proliferative state. Mutations in this gene are associated with multiple endocrine neoplasia type IV (MEN4). [provided by RefSeq, Apr 2014]
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

Related products

Western blot using GeneTex affinity purified anti-p27 antibody (GTX26547) shows detection of p27 protein in MCF7 whole cell lysate (lanes 1-3) (arrowhead). Separation was achieved using a 4-20% gradient gel. Blocking occurred using 5% BLOTTO. Primary antibody was diluted 1:500 in 1% BLOTTO. The membrane was washed and reacted with a 1:10,000 dilution of Dylight? 800 conjugated Goat anti Rabbit IgG. Molecular weight estimation was made by comparison to prestained MW markers indicated at the left (lane M). Other detection systems will yield similar results.
Product group Antibodies
GeneTex
p27 Kip1 antibodyGTX26547
ApplicationsImmunoPrecipitation, Western Blot, ELISA
TargetCDKN1B
  • SizePrice
The data was published in the journal Mol Cell Endocrinol in 2013.PMID: 23791816
Product group Antibodies
References
GeneTex
p27 Kip1 antibodyGTX27961
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry
TargetCDKN1B
  • SizePrice
Product group Antibodies
RayBiotech
Anti-p27 Antibody130-10050
ApplicationsELISA
TargetCDKN1B
  • SizePrice
RSC96 probed with CDKN1B Polyclonal Antibody, Unconjugated (bs-0742R) at 1:100 for 30 minutes followed by incubation with a conjugated secondary (PE Conjugated) (green) for 30 minutes compared to control cells (blue), secondary only (light blue) and isotype control (orange).
Product group Antibodies
References
Bioss Antibodies
CDKN1B/p27 KIP 1 Polyclonal AntibodyBS-0742R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCDKN1B
  • SizePrice
Product group Antibodies
MedChem Express
p27 KIP 1 Antibody (YA253, Rabbit)HY-P80256
TargetCDKN1B
  • SizePrice
Figure 3. Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1/CDKN1B antibody (PB9070). Overlay histogram showing SiHa cells stained with PB9070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1/CDKN1B Antibody (PB9070, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
Product group Antibodies
Boster Bio
Anti-p27 KIP 1/CDKN1B Antibody Picoband(r)PB9070-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
TargetCDKN1B
  • SizePrice
WB analysis of p27 Kip1(AA: 1-198)-hIgGFc transfected HEK293 cell lysate using GTX82990 p27 Kip1 antibody [3D8].
Product group Antibodies
GeneTex
p27 Kip1 antibody [3D8]GTX82990
ApplicationsWestern Blot, ELISA
TargetCDKN1B
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-P27KIP1 / CDKN1B (C Terminus)EB05061
ApplicationsImmunoPrecipitation, Western Blot, ELISA
TargetCDKN1B
  • SizePrice
Product group Antibodies
References
GeneTex
p27 Kip1 antibodyGTX100446
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
TargetCDKN1B
  • SizePrice
Product group Antibodies
GeneTex
p27 Kip1 antibodyGTX14243
ApplicationsWestern Blot
TargetCDKN1B
  • SizePrice