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Anti-p27 Antibody

130-10050
RayBiotech
ApplicationsELISA
Product group Antibodies
TargetCDKN1B
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Overview

  • Supplier
    RayBiotech
  • Product Name
    Anti-p27 Antibody
  • Delivery Days Customer
    16
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Gene ID1027
  • Target name
    CDKN1B
  • Target description
    cyclin dependent kinase inhibitor 1B
  • Target synonyms
    CDKN4; cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); KIP1; MEN1B; MEN4; P27KIP1
  • Host
    Rabbit
  • Protein IDP46527
  • Protein Name
    Cyclin-dependent kinase inhibitor 1B
  • Scientific Description
    Rabbit Anti-Human p27 Antibody
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 3. Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1/CDKN1B antibody (PB9070). Overlay histogram showing SiHa cells stained with PB9070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1/CDKN1B Antibody (PB9070, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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