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Mouse anti Mouse p27

C155M
Nordic-MUbio
ApplicationsImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetCDKN1B
100 ug
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Overview

  • Supplier
    Nordic-MUbio
  • Product Name
    Mouse anti Mouse p27
  • Delivery Days Customer
    7
  • Application Supplier Note
    Western Blot (5-10 microg/ml), Immunohistochemistry, Immunoprecipitation (co-precipitates cdk4)
  • Applications
    ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Western Blotting;Immunoprecipitation;Immunohistochemistry (paraffin)
  • Category Supplier
    Primary antibodies
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    DCS72
  • Conjugate
    Unconjugated
  • Gene ID1027
  • Target name
    CDKN1B
  • Target description
    cyclin dependent kinase inhibitor 1B
  • Target synonyms
    CDKN4; cyclin-dependent kinase inhibitor 1B; cyclin-dependent kinase inhibitor 1B (p27, Kip1); KIP1; MEN1B; MEN4; P27KIP1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP46527
  • Protein Name
    Cyclin-dependent kinase inhibitor 1B
  • Scientific Description
    p27Kip1; Cyclin-dependent kinase inhibitor p27; Cyclin-dependent kinase inhibitor 1B
  • Shelf life instruction
    See expiration date on vial
  • Source
    The immunogen for the anti-mouse p27 was a his-tagged recombinant full length mouse p27 protein recognizing a 27kDa protein.
  • Reactivity Supplier
    Human;Mouse;Rat;Dog
  • UNSPSC
    12352203

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Figure 3. Flow Cytometry analysis of SiHa cells using anti-p27 KIP 1/CDKN1B antibody (PB9070). Overlay histogram showing SiHa cells stained with PB9070 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-p27 KIP 1/CDKN1B Antibody (PB9070, 1 microg/1x106 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (BA1127, 5-10 microg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 microg/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.
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