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Western Blot Positive WB detected in: Mouse brain tissue, Mouse kidney tissue, Mouse liver tissue, Rat lung tissue, Rat skeletal muscle tissue All lanes: P2RX4 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 46, 41 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Mouse kidney tissue, Mouse liver tissue, Rat lung tissue, Rat skeletal muscle tissue All lanes: P2RX4 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 46, 41 kDa Observed band size: 44 kDa
Western Blot Positive WB detected in: Mouse brain tissue, Mouse kidney tissue, Mouse liver tissue, Rat lung tissue, Rat skeletal muscle tissue All lanes: P2RX4 antibody at 3ug/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 44, 46, 41 kDa Observed band size: 44 kDa

P2RX4 Antibody

CSB-PA859513LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
TargetP2RX4
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Overview

  • Supplier
    Cusabio
  • Product Name
    P2RX4 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5025
  • Target name
    P2RX4
  • Target description
    purinergic receptor P2X 4
  • Target synonyms
    ATP receptor; ATP-gated cation channel protein; P2X purinoceptor 4; P2X receptor, subunit 4; P2X4; P2X4R; purinergic receptor P2X, ligand gated ion channel, 4; purinergic receptor P2X4; purinoceptor P2X4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99571
  • Protein Name
    P2X purinoceptor 4
  • Scientific Description
    Receptor for ATP that acts as a ligand-gated ion channel. This receptor is insensitive to the antagonists PPADS and suramin.
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P2X4/P2RX4 using anti-P2X4/P2RX4 antibody (A04715-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X4/P2RX4 antigen affinity purified polyclonal antibody (Catalog # A04715-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2X4/P2RX4 at approximately 70 kDa. The expected band size for P2X4/P2RX4 is at 43 kDa.
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