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P2RX4 / P2X4 Antibody

LS-C334869
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
Product group Antibodies
TargetP2RX4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    P2RX4 / P2X4 Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 40kDa/43kDa/45kDa, while the observed MW by Western blot was 62kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IF (1:10 - 1:100), IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 40kDa/43kDa/45kDa, while the observed MW by Western blot was 62kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5025
  • Target name
    P2RX4
  • Target description
    purinergic receptor P2X 4
  • Target synonyms
    P2X4, P2X4R, P2X purinoceptor 4, ATP receptor, ATP-gated cation channel protein, P2X receptor, subunit 4, purinergic receptor P2X, ligand gated ion channel, 4, purinergic receptor P2X4, purinoceptor P2X4
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P2X4/P2RX4 using anti-P2X4/P2RX4 antibody (A04715-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X4/P2RX4 antigen affinity purified polyclonal antibody (Catalog # A04715-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2X4/P2RX4 at approximately 70 kDa. The expected band size for P2X4/P2RX4 is at 43 kDa.
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