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WB analysis of various sample lysates using GTX64406 P2X4 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64406 P2X4 antibody. Dilution : 1:1000 Loading : 25microg per lane
WB analysis of various sample lysates using GTX64406 P2X4 antibody. Dilution : 1:1000 Loading : 25microg per lane

P2X4 antibody

GTX64406
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
TargetP2RX4
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Overview

  • Supplier
    GeneTex
  • Product Name
    P2X4 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500 - 1:2000. ICC/IF: 1:10 - 1:100. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5025
  • Target name
    P2RX4
  • Target description
    purinergic receptor P2X 4
  • Target synonyms
    ATP receptor; ATP-gated cation channel protein; P2X purinoceptor 4; P2X receptor, subunit 4; P2X4; P2X4R; purinergic receptor P2X, ligand gated ion channel, 4; purinergic receptor P2X4; purinoceptor P2X4
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ99571
  • Protein Name
    P2X purinoceptor 4
  • Scientific Description
    The product of this gene belongs to the family of purinoceptors for ATP. This receptor functions as a ligand-gated ion channel with high calcium permeability. The main pharmacological distinction between the members of the purinoceptor family is the relative sensitivity to the antagonists suramin and PPADS. The product of this gene has the lowest sensitivity for these antagonists. Multiple alternatively spliced transcript variants, some protein-coding and some not protein-coding, have been found for this gene. [provided by RefSeq, Feb 2012]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P2X4/P2RX4 using anti-P2X4/P2RX4 antibody (A04715-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human SH-SY5Y whole cell lysates, Lane 4: human MCF-7 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2X4/P2RX4 antigen affinity purified polyclonal antibody (Catalog # A04715-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2X4/P2RX4 at approximately 70 kDa. The expected band size for P2X4/P2RX4 is at 43 kDa.
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