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ProductsBack/P2Y12 antibody [HL2829]

P2Y12 antibody [HL2829]

GTX640100
GeneTex
ApplicationsImmunoFluorescence, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetP2RY12
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Overview

  • Supplier
    GeneTex
  • Product Name
    P2Y12 antibody [HL2829]
  • Delivery Days Customer
    7
  • Applications
    ImmunoFluorescence, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL2829
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID64805
  • Target name
    P2RY12
  • Target description
    purinergic receptor P2Y12
  • Target synonyms
    ADP-glucose receptor; ADPG-R; BDPLT8; Gi-coupled ADP receptor HORK3; G-protein coupled receptor SP1999; HORK3; P2T(AC); P2Y purinoceptor 12; P2Y(12)R; P2Y(AC); P2Y(ADP); P2Y(cyc); P2Y12; P2Y12 platelet ADP receptor; purinergic receptor P2RY12; purinergic receptor P2Y, G-protein coupled, 12; putative G-protein coupled receptor; SP1999
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H244
  • Protein Name
    P2Y purinoceptor 12
  • Scientific Description
    The product of this gene belongs to the family of G-protein coupled receptors. This family has several receptor subtypes with different pharmacological selectivity, which overlaps in some cases, for various adenosine and uridine nucleotides. This receptor is involved in platelet aggregation, and is a potential target for the treatment of thromboembolisms and other clotting disorders. Mutations in this gene are implicated in bleeding disorder, platelet type 8 (BDPLT8). Alternative splicing results in multiple transcript variants of this gene. [provided by RefSeq, Jul 2013]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of P2RY12 using anti-P2RY12 antibody (M01136). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SH-SY5Y whole cell lysates, Lane 3: human U-87 MG whole cell lysates, Lane 4: human U251 whole cell lysates, Lane 5: rat brain tissue lysates, Lane 6: rat C6 whole cell lysates, Lane 7: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P2RY12 antigen affinity purified monoclonal antibody (Catalog # M01136) at 1:500 overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:500 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P2RY12 at approximately 50 kDa. The expected band size for P2RY12 is at 39 kDa.
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IHC-Fr analysis of mouse brain tissue using GTX54796 P2Y12 antibody. The region shown in this picture is the entorhinal cortex in mouse brain. The P2Y12 positive fibers (arrows) run from the corpus callosum (triangles) into the deep layers of the entorhinal cortex. Sytox green is used as the counterstain.
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Raw264.7 cell lysates probed with P2Y12 Polyclonal Antibody, Unconjugated (bs-12072R) at 1:1000 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:20000 for 60 min at 37˚C.
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