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MCF-7 cells were stained with p38 MAPK Polyclonal Antibody, Unconjugated(bs-0637R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
MCF-7 cells were stained with p38 MAPK Polyclonal Antibody, Unconjugated(bs-0637R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.
MCF-7 cells were stained with p38 MAPK Polyclonal Antibody, Unconjugated(bs-0637R) at 1:500 in PBS and incubated for two hours at 37°C followed by Goat Anti-Rabbit IgG (H+L) Cy3 conjugated secondary antibody. DAPI staining of the nucleus was done and then detected.

P38 MAPK Polyclonal Antibody

BS-0637R
Bioss Antibodies
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
DownloadDatasheet
Product group Antibodies
TargetMAPK14
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Overview

  • Supplier
    Bioss Antibodies
  • Product Name
    P38 MAPK Polyclonal Antibody
  • Delivery Days Customer
    16
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    WB(1:300-5000), ELISA(1:500-1000), FCM(1:20-100), IHC-P(1:200-400), IHC-F(1:100-500), IF(IHC-P)(1:50-200), IF(IHC-F)(1:50-200), IF(ICC)(1:50-200)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 ug/ul
  • Conjugate
    Unconjugated
  • Gene ID1432
  • Target name
    MAPK14
  • Target description
    mitogen-activated protein kinase 14
  • Target synonyms
    CSAID-binding protein; CSBP; CSBP1; CSBP2; CSPB1; cytokine suppressive anti-inflammatory drug binding protein; EXIP; MAP kinase 14; MAP kinase Mxi2; MAP kinase p38 alpha; MAX-interacting protein 2; mitogen-activated protein kinase 14; mitogen-activated protein kinase p38 alpha; Mxi2; p38; p38 MAP kinase; p38 mitogen activated protein kinase; p38ALPHA; p38alpha Exip; PRKM14; PRKM15; RK; SAPK2A; stress-activated protein kinase 2A
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ16539
  • Protein Name
    Mitogen-activated protein kinase 14
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

References

  • Lactobacillus plantarum NA136 ameliorates nonalcoholic fatty liver disease by modulating gut microbiota, improving intestinal barrier integrity, and attenuating inflammation. Zhao Z et al., 2020 Jun, Appl Microbiol Biotechnol
    Read more
  • MicroRNA-379 mediates pigmentation, migration and proliferation of melanocytes by targeting the insulin-like growth factor 1 receptor. Liu B et al., 2020 May, Exp Dermatol
    Read more
  • Double-component diazeniumdiolate derivatives as anti-cancer agents. Ji X et al., 2020 Apr 15, Bioorg Med Chem
    Read more
  • Combining transcatheter arterial embolization with iodized oil containing Apatinib inhibits HCC growth and metastasis. Zhou C et al., 2020 Feb 19, Sci Rep
    Read more
  • Down-regulation of Suv39h1 attenuates neointima formation after carotid artery injury in diabetic rats. Zhang J et al., 2020 Jan, J Cell Mol Med
    Read more
  • SPARC promotes self-renewal of limbal epithelial stem cells and ocular surface restoration through JNK and p38-MAPK signaling pathways. Zhu J et al., 2020 Jan, Stem Cells
    Read more
  • Paeonol antagonizes oncogenesis of osteosarcoma by inhibiting the function of TLR4/MAPK/NF-kappaB pathway. Zhou J et al., 2020 Jan, Acta Histochem
    Read more
  • Vitamin B5 inhibit RANKL induced osteoclastogenesis and ovariectomy induced osteoporosis by scavenging ROS generation. Ma Q et al., 2019, Am J Transl Res
    Read more
  • Withanolides, Extracted from Datura Metel L. Inhibit Keratinocyte Proliferation and Imiquimod-Induced Psoriasis-Like Dermatitis via the STAT3/P38/ERK1/2 Pathway. Li T et al., 2019 Jul 17, Molecules
    Read more
  • Chrysophanol attenuates airway inflammation and remodeling through nuclear factor-kappa B signaling pathway in asthma. Song G et al., 2019 Oct, Phytother Res
    Read more

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Figure 1. Western blot analysis of P38 Alpha/MAPK14 using anti-P38 Alpha/MAPK14 antibody (A00176-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human SW620 whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: rat lung tissue lysates, Lane 5: rat heart tissue lysates, Lane 6: mouse lung tissue lysates, Lane 7: mouse heart tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P38 Alpha/MAPK14 antigen affinity purified polyclonal antibody (Catalog # A00176-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P38 Alpha/MAPK14 at approximately 41 kDa. The expected band size for P38 Alpha/MAPK14 is at 41 kDa.
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