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P4HB Antibody Pair

CSB-EAP00254
Cusabio
ApplicationsELISA
Product group Antibodies
ReactivityBovine, Canine, Human, Mouse, Porcine, Sheep
TargetP4HB
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Overview

  • Supplier
    Cusabio
  • Product Name
    P4HB Antibody Pair
  • Delivery Days Customer
    9
  • Application Supplier Note
    We recommend using the capture antibody at a concentration of 0.9ug/ml and the detection antibody at a concentration of 0.25ug/ml.Optimal dilutions should be determined experimentally by the researcher.
  • Applications
    ELISA
  • Certification
    Research Use Only
  • Conjugate
    Biotin
  • Gene ID5034
  • Target name
    P4HB
  • Target description
    prolyl 4-hydroxylase subunit beta
  • Target synonyms
    CLCRP1, DSI, ERBA2L, GIT, P4Hbeta, PDI, PDIA1, PHDB, PO4DB, PO4HB, PROHB, protein disulfide-isomerase, cellular thyroid hormone-binding protein, collagen prolyl 4-hydroxylase beta, glutathione-insulin transhydrogenase, p55, procollagen-proline, 2-oxoglutarate 4-dioxygenase (proline 4-hydroxylase), beta polypeptide, prolyl 4-hydroxylase, beta polypeptide, protein disulfide isomerase family A, member 1, protein disulfide isomerase-associated 1, protein disulfide isomerase/oxidoreductase, protocollagen hydroxylase, testicular secretory protein Li 32, thyroid hormone-binding protein p55
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP07237
  • Protein Name
    Protein disulfide-isomerase
  • Reactivity
    Bovine, Canine, Human, Mouse, Porcine, Sheep
  • UNSPSC
    41116163

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Figure 1. Western blot analysis of P4HB using anti-P4HB antibody (A02335-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PANC-1 whole cell lysates, Lane 3: human MCF-7 whole cell lysates, Lane 4: human COLO-320 whole cell lysates, Lane 5: human HEK293 whole cell lysates, Lane 6: human HepG2 whole cell lysates, Lane 7: monkey lung tissue lysates, Lane 8: monkey liver tissue lysates, Lane 9: rat liver tissue lysates, Lane 10: mouse liver tissue lysates, Lane 11: mouse NIH/3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-P4HB antigen affinity purified polyclonal antibody (Catalog # A02335-1) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for P4HB at approximately 57KD. The expected band size for P4HB is at 57KD.
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