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ProductsBack/p75 NGF Receptor / CD271 antibody [2F1C2]
FACS analysis of EC cells using GTX83360 p75 NGF Receptor / CD271 antibody [2F1C2]. Green : p75 NGF Receptor / CD271 Purple : negative control
FACS analysis of EC cells using GTX83360 p75 NGF Receptor / CD271 antibody [2F1C2]. Green : p75 NGF Receptor / CD271 Purple : negative control
FACS analysis of EC cells using GTX83360 p75 NGF Receptor / CD271 antibody [2F1C2]. Green : p75 NGF Receptor / CD271 Purple : negative control

p75 NGF Receptor / CD271 antibody [2F1C2]

GTX83360
GeneTex
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
DownloadDatasheet
Product group Antibodies
TargetNGFR
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Overview

  • Supplier
    GeneTex
  • Product Name
    p75 NGF Receptor / CD271 antibody [2F1C2]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1/500 - 1/2000. ICC/IF: 1/200 - 1/1000. FACS: 1/200 - 1/400. ELISA: 1/10000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    Flow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    2F1C2
  • Conjugate
    Unconjugated
  • Gene ID4804
  • Target name
    NGFR
  • Target description
    nerve growth factor receptor
  • Target synonyms
    CD271; Gp80-LNGFR; low affinity neurotrophin receptor p75NTR; low-affinity nerve growth factor receptor; NGF receptor; p75 ICD; p75(NTR); p75NTR; TNFR superfamily, member 16; TNFRSF16; tumor necrosis factor receptor superfamily member 16
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP08138
  • Protein Name
    Tumor necrosis factor receptor superfamily member 16
  • Scientific Description
    Nerve growth factor receptor contains an extracellular domain containing four 40-amino acid repeats with 6 cysteine residues at conserved positions followed by a serine/threonine-rich region, a single transmembrane domain, and a 155-amino acid cytoplasmic domain. The cysteine-rich region contains the nerve growth factor binding domain. [provided by RefSeq, Jul 2008]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of p75 NGF Receptor/NGFR using anti-p75 NGF Receptor/NGFR antibody (A01187). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: human Sw620 whole cell lysates, Lane 3: human Caco-2 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-p75 NGF Receptor/NGFR antigen affinity purified polyclonal antibody (Catalog # A01187) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for p75 NGF Receptor/NGFR at approximately 45KD,75KD. The expected band size for p75 NGF Receptor/NGFR is at 45KD,75KD.
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ICC/IF analysis of U-251 MG cells using GTX04906 p75 NGF Receptor / CD271 antibody. Orange : Primary antibody Blue : DAPI Dilution : 1:100
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