More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PAH Antibody
Western Blot analysis of HepG2 cells using PAH Polyclonal Antibody
Western Blot analysis of HepG2 cells using PAH Polyclonal Antibody
Western Blot analysis of HepG2 cells using PAH Polyclonal Antibody

PAH Antibody

CSB-PA003698
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPAH
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    Cusabio
  • Product Name
    PAH Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID5053
  • Target name
    PAH
  • Target description
    phenylalanine hydroxylase
  • Target synonyms
    PH, PKU, PKU1, phenylalanine-4-hydroxylase, phe-4-monooxygenase, phenylalanine 4-monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP00439
  • Protein Name
    Phenylalanine-4-hydroxylase
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
TBX5 AntibodyCAB15590
ReactivityHuman
TargetPAH
  • SizePrice
Figure 1. Western blot analysis of PAH using anti-PAH antibody (A00761-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAH antigen affinity purified polyclonal antibody (Catalog # A00761-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAH at approximately 45 kDa. The expected band size for PAH is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PAH Antibody Picoband(r)A00761-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPAH
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PAH Antibody144-01559
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPAH
  • SizePrice
Anti-PAH Antibody
Product group Antibodies
Antibodies.com
Anti-PAH AntibodyA96438
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
PAH Antibody (YA1696, Rabbit)HY-P81951
TargetPAH
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-Phenylalanine HydroxylaseEB10324
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Rat
TargetPAH
  • SizePrice
Immunohistochemistry analysis in human liver and pancreas tissues using HPA028407 antibody. Corresponding PAH RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-PAH AntibodyHPA028407
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPAH
  • SizePrice
Product group Antibodies
LifeSpan BioSciences
PH / PAH AntibodyLS-C331535
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse
TargetPAH
  • SizePrice
A431 lysates probed with PAH/PH4H Polyclonal Antibody, Unconjugated (bs-21005R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
Bioss
PAH Polyclonal AntibodyBS-21005R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Rat
TargetPAH
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAH antibody [N1C2] (GTX101290) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Product group Antibodies
GeneTex
PAH antibody [N1C2]GTX101290
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetPAH
  • SizePrice