Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAH antibody [N1C2] (GTX101290) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
![PAH antibody [N1C2] detects PAH protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAH stained by PAH antibody [N1C2] (GTX101290) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).](https://www.genetex.com/upload/website/prouct_img/normal/GTX101290/GTX101290_43712_20200806_ICC_IF_w_23060100_578.webp "PAH antibody [N1C2] detects PAH protein at cytoplasm by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PAH stained by PAH antibody [N1C2] (GTX101290) diluted at 1:500. Blue: Fluoroshield with DAPI (GTX30920).")
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAH antibody [N1C2] (GTX101290) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
PAH antibody [N1C2]
GTX101290
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPAH
Overview
- SupplierGeneTex
- Product NamePAH antibody [N1C2]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1.76 mg/ml
- ConjugateUnconjugated
- Gene ID5053
- Target namePAH
- Target descriptionphenylalanine hydroxylase
- Target synonymsPH, PKU, PKU1, phenylalanine-4-hydroxylase, phe-4-monooxygenase, phenylalanine 4-monooxygenase
- HostRabbit
- IsotypeIgG
- Protein IDP00439
- Protein NamePhenylalanine-4-hydroxylase
- Scientific DescriptionThis gene encodes a member of the biopterin-dependent aromatic amino acid hydroxylase protein family. The encoded phenylalanine hydroxylase enzyme hydroxylates phenylalanine to tyrosine and is the rate-limiting step in phenylalanine catabolism. Deficiency of this enzyme activity results in the autosomal recessive disorder phenylketonuria. [provided by RefSeq, Aug 2017]
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC41116161
Datasheet
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