More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PH / PAH Antibody

PH / PAH Antibody

LS-C331535
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPAH
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PH / PAH Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    The predicted MW is 51kDa, while the observed MW by Western blot was 55kDa.
  • Applications
    Western Blot, ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:50 - 1:200), WB (1:500 - 1:2000) The predicted MW is 51kDa, while the observed MW by Western blot was 55kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5053
  • Target name
    PAH
  • Target description
    phenylalanine hydroxylase
  • Target synonyms
    PH, PKU, PKU1, phenylalanine-4-hydroxylase, phe-4-monooxygenase, phenylalanine 4-monooxygenase
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

Go to our frequently asked questions

Related products

Product group Antibodies
Assay Genie
TBX5 AntibodyCAB15590
ReactivityHuman
TargetPAH
  • SizePrice
Western Blot analysis of HepG2 cells using PAH Polyclonal Antibody
Product group Antibodies
Cusabio
PAH AntibodyCSB-PA003698
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPAH
  • SizePrice
Figure 1. Western blot analysis of PAH using anti-PAH antibody (A00761-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human CACO-2 whole cell lysates, Lane 3: rat kidney tissue lysates, Lane 4: rat liver tissue lysates, Lane 5: mouse kidney tissue lysates, Lane 6: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAH antigen affinity purified polyclonal antibody (Catalog # A00761-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAH at approximately 45 kDa. The expected band size for PAH is at 52 kDa.
Product group Antibodies
Boster Bio
Anti-PAH Antibody Picoband(r)A00761-1-CARRIER-FREE
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPAH
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PAH Antibody144-01559
ApplicationsWestern Blot
ReactivityHuman, Mouse, Rat
TargetPAH
  • SizePrice
Anti-PAH Antibody
Product group Antibodies
Antibodies.com
Anti-PAH AntibodyA96438
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
  • SizePrice
Product group Antibodies
MedChem Express
PAH Antibody (YA1696, Rabbit)HY-P81951
TargetPAH
  • SizePrice
Product group Antibodies
Everest Biotech
Goat anti-Phenylalanine HydroxylaseEB10324
ApplicationsWestern Blot, ELISA
ReactivityBovine, Canine, Human, Mouse, Rat
TargetPAH
  • SizePrice
Immunohistochemistry analysis in human liver and pancreas tissues using HPA028407 antibody. Corresponding PAH RNA-seq data are presented for the same tissues.
Product group Antibodies
Atlas Antibodies
Anti-PAH AntibodyHPA028407
ApplicationsWestern Blot, ImmunoHistoChemistry
ReactivityHuman
TargetPAH
  • SizePrice
A431 lysates probed with PAH/PH4H Polyclonal Antibody, Unconjugated (bs-21005R) at 1:300 dilution and 4˚C overnight incubation. Followed by conjugated secondary antibody incubation at 1:10000 for 60 min at 37˚C.
Product group Antibodies
Bioss
PAH Polyclonal AntibodyBS-21005R
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityBovine, Human, Mouse, Rat
TargetPAH
  • SizePrice
Various whole cell extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PAH antibody [N1C2] (GTX101290) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
Product group Antibodies
GeneTex
PAH antibody [N1C2]GTX101290
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
ReactivityHuman
TargetPAH
  • SizePrice