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Western blot All lanes: PAIP1 antibody at 8ug/ml Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 46, 43 kDa Observed band size: 54 kDa
Western blot All lanes: PAIP1 antibody at 8ug/ml Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 46, 43 kDa Observed band size: 54 kDa
Western blot All lanes: PAIP1 antibody at 8ug/ml Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 46, 43 kDa Observed band size: 54 kDa

PAIP1 Antibody

CSB-PA880930ESR2HU
Cusabio
ApplicationsWestern Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPAIP1
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Overview

  • Supplier
    Cusabio
  • Product Name
    PAIP1 Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID10605
  • Target name
    PAIP1
  • Target description
    poly(A) binding protein interacting protein 1
  • Target synonyms
    polyadenylate-binding protein-interacting protein 1, PABC1-interacting protein 1, PABP-interacting protein 1, PAIP-1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDQ9H074
  • Protein Name
    Polyadenylate-binding protein-interacting protein 1
  • Scientific Description
    Acts as a coactivator in the regulation of translation initiation of poly(A)-containing mRNAs. Its stimulatory activity on translation is mediated via its action on PABPC1. Competes with PAIP2 for binding to PABPC1. Its association with EIF4A and PABPC1 may potentiate contacts between mRNA termini. May also be involved in translationally coupled mRNA turnover. Implicated with other RNA-binding proteins in the cytoplasmic deadenylation/translational and decay interplay of the FOS mRNA mediated by the major coding-region determinant of instability (mCRD) domain.
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PAIP1 using anti-PAIP1 antibody (A07792-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAIP1 antigen affinity purified polyclonal antibody (Catalog # A07792-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAIP1 at approximately 45 kDa. The expected band size for PAIP1 is at 54 kDa.
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