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PAIP1 Antibody

LS-C831591
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry
Product group Antibodies
TargetPAIP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PAIP1 Antibody
  • Delivery Days Customer
    23
  • Applications
    ImmunoHistoChemistry
  • Applications Supplier
    IHC (1:100 - 1:300)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID10605
  • Target name
    PAIP1
  • Target description
    poly(A) binding protein interacting protein 1
  • Target synonyms
    PABC1-interacting protein 1; PABP-interacting protein 1; PAIP-1; polyadenylate-binding protein-interacting protein 1
  • Host
    Rabbit
  • Isotype
    IgG
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Western blot All lanes: PAIP1 antibody at 8ug/ml Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 54, 46, 43 kDa Observed band size: 54 kDa
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Figure 1. Western blot analysis of PAIP1 using anti-PAIP1 antibody (A07792-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human PC-3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAIP1 antigen affinity purified polyclonal antibody (Catalog # A07792-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAIP1 at approximately 45 kDa. The expected band size for PAIP1 is at 54 kDa.
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