More than 130 suppliers
ISO certified
In-house tech support
we Connect you
Bio-Connect
ProductsBack/PARP antibody [HL1364]
Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Untreated (–) and treated (+) HCT-116 whole cell extract (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP antibody [HL1364] (GTX636804) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.

PARP antibody [HL1364]

GTX636804
GeneTex
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse
TargetPARP1
Sign in to order and to see your custom pricing.
Large volume orders?
Order with a bulk request
More than 130 suppliers
ISO certified
In-house tech support

Overview

  • Supplier
    GeneTex
  • Product Name
    PARP antibody [HL1364]
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:5000-1:20000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    HL1364
  • Concentration
    1.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID142
  • Target name
    PARP1
  • Target description
    poly(ADP-ribose) polymerase 1
  • Target synonyms
    ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1, poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase), ADP-ribosyltransferase NAD(+), ADP-ribosyltransferase diphtheria toxin-like 1, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, poly (ADP-ribose) polymerase family, member 1, poly(ADP-ribose) synthetase, poly(ADP-ribosyl)transferase, poly[ADP-ribose] synthase 1, protein poly-ADP-ribosyltransferase PARP1
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP09874
  • Protein Name
    Poly [ADP-ribose] polymerase 1
  • Scientific Description
    This gene encodes a chromatin-associated enzyme, poly(ADP-ribosyl)transferase, which modifies various nuclear proteins by poly(ADP-ribosyl)ation. The modification is dependent on DNA and is involved in the regulation of various important cellular processes such as differentiation, proliferation, and tumor transformation and also in the regulation of the molecular events involved in the recovery of cell from DNA damage. In addition, this enzyme may be the site of mutation in Fanconi anemia, and may participate in the pathophysiology of type I diabetes. [provided by RefSeq, Jul 2008]
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

Datasheet

Download Datasheet

FAQ

Go to our frequently asked questions

Related products

Figure 1. Western blot analysis of PARP1 using anti-PARP1 antibody (A00122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (Catalog # A00122-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1 at approximately 113 kDa. The expected band size for PARP1 is at 113 kDa.
Product group Antibodies
Boster Bio
Anti-PARP1 Antibody Picoband(r)A00122-2-CARRIER-FREE
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry
ReactivityHuman
TargetPARP1
  • SizePrice
Product group Antibodies
RayBiotech
Anti-PARP1 Antibody144-00942
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry
ReactivityHuman, Mouse, Rat
TargetPARP1
  • SizePrice
Immunohistochemical staining of human tonsil shows strong nuclear positivity in lymphoid cells.
Product group Antibodies
Atlas Antibodies
Anti-PARP1 AntibodyAMAB90959
ApplicationsWestern Blot, ImmunoCytoChemistry, ImmunoHistoChemistry
ReactivityHuman
TargetPARP1
  • SizePrice
Product group Antibodies
Biomatik
PARP1 Polyclonal AntibodyCAC15893
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry
TargetPARP1
  • SizePrice
Product group Antibodies
Assay Genie
PARP1 AntibodyCAB0010
ReactivityHuman
TargetPARP1
  • SizePrice
Paraformaldehyde-fixed, paraffin embedded mouse brain; Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with PARP1 Polyclonal Antibody, Unconjugated (bs-2138R) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.
Product group Antibodies
References
Bioss
PARP1 Polyclonal AntibodyBS-2138R
ApplicationsFlow Cytometry, ImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityHuman
TargetPARP1
  • SizePrice
Western blot analysis of 1) Hela, 2) 293T, 3) Jurkat, diluted at 1:2000.
Product group Antibodies
Cusabio
PARP1 Monoclonal AntibodyCSB-MA000290
ApplicationsWestern Blot, ELISA
ReactivityHuman
TargetPARP1
  • SizePrice
FACS analysis of Jurkat cells using GTX83005 PARP antibody [7A10]. Green : PARP Purple : negative control
Product group Antibodies
GeneTex
PARP antibody [7A10]GTX83005
ApplicationsFlow Cytometry, Western Blot, ELISA
ReactivityHuman
TargetPARP1
  • SizePrice
WB analysis of NIH/3T3 cells treated with etoposide 25uM (1h) lysate using GTX86935 PARP (cleaved Gly215) antibody. The lane on the right is blocked with the synthesized peptide.
Product group Antibodies
GeneTex
PARP (cleaved Gly215) antibodyGTX86935
ApplicationsWestern Blot
ReactivityHuman, Mouse
TargetPARP1
  • SizePrice
Product group Antibodies
MedChem Express
PARP1 Antibody (YA246, Rabbit)HY-P80263
TargetPARP1
  • SizePrice