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ProductsBack/PARP1 Mouse Monoclonal Antibody [Clone ID: 7A10]
Western blot analysis using PARP antibody Cat.-No AM06413SU-N against Jurkat (Lane 1), K562 (Lane 2), Hela (Lane 3), Raji (Lane 4), THP-1 (Lane 5) and SW620 (Lane 6) cell lysate.
Western blot analysis using PARP antibody Cat.-No AM06413SU-N against Jurkat (Lane 1), K562 (Lane 2), Hela (Lane 3), Raji (Lane 4), THP-1 (Lane 5) and SW620 (Lane 6) cell lysate.
Western blot analysis using PARP antibody Cat.-No AM06413SU-N against Jurkat (Lane 1), K562 (Lane 2), Hela (Lane 3), Raji (Lane 4), THP-1 (Lane 5) and SW620 (Lane 6) cell lysate.

PARP1 Mouse Monoclonal Antibody [Clone ID: 7A10]

AM06413SU-N
OriGene
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
ReactivityHuman
TargetPARP1
Price on request
100 ul
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Overview

  • Supplier
    OriGene
  • Product Name
    PARP1 Mouse Monoclonal Antibody [Clone ID: 7A10]
  • Delivery Days Customer
    14
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    7A10
  • Gene ID142
  • Target name
    PARP1
  • Target description
    poly(ADP-ribose) polymerase 1
  • Target synonyms
    ADPRT, ADPRT 1, ADPRT1, ARTD1, PARP, PARP-1, PARS, PPOL, Poly-PARP, pADPRT-1, poly [ADP-ribose] polymerase 1, ADP-ribosyltransferase (NAD+; poly (ADP-ribose) polymerase), ADP-ribosyltransferase NAD(+), ADP-ribosyltransferase diphtheria toxin-like 1, DNA ADP-ribosyltransferase PARP1, NAD(+) ADP-ribosyltransferase 1, poly (ADP-ribose) polymerase family, member 1, poly(ADP-ribose) synthetase, poly(ADP-ribosyl)transferase, poly[ADP-ribose] synthase 1, protein poly-ADP-ribosyltransferase PARP1
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP09874
  • Protein Name
    Poly [ADP-ribose] polymerase 1
  • Scientific Description
    PARP1 mouse monoclonal antibody, clone 7A10, Ascites
  • Reactivity
    Human
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

MSDS

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Figure 1. Western blot analysis of PARP1 using anti-PARP1 antibody (A00122-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human A549 whole cell lysates, Lane 4: human Hela whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP1 antigen affinity purified polyclonal antibody (Catalog # A00122-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP1 at approximately 113 kDa. The expected band size for PARP1 is at 113 kDa.
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