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Immunofluorescence analysis of MCF-7 cells using PARP3 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using PARP3 Polyclonal Antibody
Immunofluorescence analysis of MCF-7 cells using PARP3 Polyclonal Antibody

PARP3 Polyclonal Antibody

E-AB-61629
Elabscience
Product group Antibodies
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Overview

  • Supplier
    Elabscience
  • Product Name
    PARP3 Polyclonal Antibody
  • Delivery Days Customer
    12
  • Certification
    Research Use Only
  • Scientific Description
    The protein encoded by this gene belongs to the PARP family. These enzymes modify nuclear proteins by poly-ADP-ribosylation, which is required for DNA repair, regulation of apoptosis, and maintenance of genomic stability. This gene encodes the poly(ADP-ribosyl)transferase 3, which is preferentially localized to the daughter centriole throughout the cell cycle. Alternatively spliced transcript variants encoding different isoforms have been identified.
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PARP3 using anti-PARP3 antibody (A03762-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: monkey COS-7 whole cell lysates, Lane 2: mouse kidney tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PARP3 antigen affinity purified polyclonal antibody (Catalog # A03762-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PARP3 at approximately 60 kDa. The expected band size for PARP3 is at 60 kDa.
Product group Antibodies
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ReactivityHuman, Monkey, Mouse, Rat
TargetPARP3
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Product group Antibodies
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Product group Antibodies
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TargetPARP3
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Product group Antibodies
Bioss
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ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
ReactivityEquine, Human, Mouse, Rabbit, Rat
TargetPARP3
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Product group Antibodies
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Immunohistochemical staining of human urinary bladder shows moderate positivity in nucleoli in urothelial cells.
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TargetPARP3
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Goat anti-PARP3 AntibodyEB09029
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Various whole cell extracts (30 μg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with PARP3 antibody [HL1509] (GTX636983) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
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PARP3 antibody [HL1509]GTX636983
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TargetPARP3
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