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PathPlus(tm) APOBEC3B Antibody

LS-B12051
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetAPOBEC3B
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) APOBEC3B Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. For Immunoflorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 42 kDa Observed: 48 kDa. ELISA, IF (20 µg/ml), IHC-P (5 - 10 µg/ml), WB (1 - 2 µg/ml) APOBEC3B antibody can be used for detection of APOBEC3B by Western blot at 1 - 2 ug/ml. Antibody can also be used for Immunohistochemistry at 5 ug/mL. For Immunoflorescence start at 20 ug/mL. Antibody validated: Western Blot in rat samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 42 kDa Observed: 48 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID9582
  • Target name
    APOBEC3B
  • Target description
    apolipoprotein B mRNA editing enzyme catalytic subunit 3B
  • Target synonyms
    A3B, APOBEC1L, ARCD3, ARP4, DJ742C19.2, PHRBNL, bK150C2.2, DNA dC->dU-editing enzyme APOBEC-3B, apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like 3B, cytidine deaminase, phorbolin 2, phorbolin 3, phorbolin-1-related protein, phorbolin-2/3, probable DNA dC->dU-editing enzyme APOBEC-3B
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of APOBEC3B using anti-APOBEC3B antibody (A01088-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human THP-1 whole cell lysates, Lane 4: human HEK293 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-APOBEC3B antigen affinity purified polyclonal antibody (Catalog # A01088-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for APOBEC3B at approximately 35 kDa. The expected band size for APOBEC3B is at 46 kDa.
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