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ProductsBack/PathPlus(tm) ARG1 / Arginase 1 Antibody (clone 4E6)

PathPlus(tm) ARG1 / Arginase 1 Antibody (clone 4E6)

LS-B13223
LifeSpan BioSciences
ApplicationsFlow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetARG1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) ARG1 / Arginase 1 Antibody (clone 4E6)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IHC, IHC-P (10 µg/ml), WB (1:1000 - 1:2000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    4E6
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID383
  • Target name
    ARG1
  • Target description
    arginase 1
  • Target synonyms
    arginase-1, arginase, liver, liver-type arginase, type I arginase
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of ARG1 using anti-ARG1 antibody (A01106). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human HCCP tissue lysates, Lane 2: monkey liver tissue lysates, Lane 3: rat liver tissue lysates, Lane 4: mouse liver tissue lysates, Lane 5: rat testis tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-ARG1 antigen affinity purified polyclonal antibody (Catalog # A01106) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for ARG1 at approximately 37 kDa. The expected band size for ARG1 is at 35 kDa.
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