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ProductsBack/PathPlus(tm) BTLA / CD272 Antibody (clone 3D11)

PathPlus(tm) BTLA / CD272 Antibody (clone 3D11)

LS-B16344
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetBTLA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) BTLA / CD272 Antibody (clone 3D11)
  • Delivery Days Customer
    14
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:100), IHC-P (10 µg/ml), WB (1:4000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3D11
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID151888
  • Target name
    BTLA
  • Target description
    B and T lymphocyte associated
  • Target synonyms
    BTLA1, CD272, B- and T-lymphocyte attenuator, B- and T-lymphocyte-associated protein
  • Host
    Mouse
  • Isotype
    IgG2b
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of CD272 using anti-CD272 antibody (A03149). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HEK293 whole cell lysate, Lane 2: human Jurkat whole cell lysate, Lane 3: human CCRF-CEM whole cell lysate, Lane 4: mouse thymus tissue lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD272 antigen affinity purified polyclonal antibody (Catalog # A03149) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD272 at approximately 65KD. The expected band size for CD272 is at 33KD.
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