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ProductsBack/PathPlus(tm) CANX / Calnexin Antibody (aa200-250)

PathPlus(tm) CANX / Calnexin Antibody (aa200-250)

LS-B4403
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Chicken, Equine, Human, Mammals, Monkey, Mouse, Porcine, Rabbit
TargetCANX
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) CANX / Calnexin Antibody (aa200-250)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot: Approx. 100kD band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kD according to Human NP_001737.1 and 67.2kD according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1 ug/ml.. IF (5 µg/ml), IHC-P (5 µg/ml), Peptide-ELISA (1:32000), WB (0.3 - 1 µg/ml) Western Blot: Approx. 100kD band observed in Mouse Heart, Human Brain (Cerebellum), NIH3T3 and HeLa lysates (calculated MW of 67.6kD according to Human NP_001737.1 and 67.2kD according to Mouse NP_001103969.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Oliver et al., J Biol Chem. 1996 Jun 7;271(23):13691-6., PMID: 8662691). Recommended concentration: 0.3-1 ug/ml.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID821
  • Target name
    CANX
  • Target description
    calnexin
  • Target synonyms
    CNX, IP90, P90, calnexin, epididymis secretory sperm binding protein, major histocompatibility complex class I antigen-binding protein p88
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Chicken, Equine, Human, Mammals, Monkey, Mouse, Porcine, Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Calnexin/CANX using anti-Calnexin/CANX antibody (A03372-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human U-937 whole cell lysates, Lane 3: human placenta tissue lysates, Lane 4: human THP-1 whole cell lysates, Lane 5: mouse lung tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Calnexin/CANX antigen affinity purified polyclonal antibody (Catalog # A03372-2) at 0.25 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Calnexin/CANX at approximately 97 kDa. The expected band size for Calnexin/CANX is at 97 kDa.
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