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PathPlus(tm) COL3A1 / Collagen III Antibody

LS-B16144
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetCOL3A1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) COL3A1 / Collagen III Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) Citrate buffer of pH 6.0 Recommended IHC controls tissues are Skin. Resulting localization is Cytoplasmic
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:200 - 1:400), IHC-P (1:50), WB (1:200 - 1:400) Use Heat-induced epitope retrieval (HIER) Citrate buffer of pH 6.0 Recommended IHC controls tissues are Skin. Resulting localization is Cytoplasmic
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Conjugate
    Unconjugated
  • Gene ID1281
  • Target name
    COL3A1
  • Target description
    collagen type III alpha 1 chain
  • Target synonyms
    EDS4A, EDSVASC, PMGEDSV, collagen alpha-1(III) chain, Ehlers-Danlos syndrome type IV, autosomal dominant, alpha-1 type III collagen, alpha1 (III) collagen, collagen, fetal, collagen, type III, alpha 1
  • Host
    Mouse
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Collagen III/COL3A1 using anti-Collagen III/COL3A1 antibody (A00788-3). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse skin tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Collagen III/COL3A1 antigen affinity purified polyclonal antibody (Catalog # A00788-3) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Collagen III/COL3A1 at approximately 200 kDa. The expected band size for Collagen III/COL3A1 is at 139 kDa.
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