PathPlus(tm) FGF2 / Basic FGF Antibody

LifeSpan BioSciences

PathPlus(tm) FGF2 / Basic FGF Antibody

23

Human FGF-basic

Immunohistochemistry: LS-B7426 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-basic (0.3 ng/ml), a concentration of 0.25-0.40 ug/ml of this antibody is required. ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (LS-C104313) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-basic. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.. ELISA (0.5 - 2 µg/ml), IHC, IHC-P (5 µg/ml), WB (0.1 - 0.2 µg/ml) Immunohistochemistry: LS-B7426 in an IHC-plus antibody, validated by our pathologists for use in IHC against formalin-fixed, paraffin-embedded human tissues (IHC validation procedure). A concentration of 5 ug/ml is recommended. Neutralization: To yield one-half maximal inhibition [ND] of the biological activity of Human FGF-basic (0.3 ng/ml), a concentration of 0.25-0.40 ug/ml of this antibody is required. ELISA: To detect Human FGF-basic by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5-2 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Biotinylated Anti-Human FGF-basic (LS-C104313) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of recombinant Human FGF-basic. Western Blot: To detect Human FGF-basic by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant Human FGF-basic is 1.5-3 ng/lane, under either reducing or non-reducing conditions.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

Unconjugated

...

Lyophilized

FGF2

fibroblast growth factor 2

basic fibroblast growth factor bFGF; BFGF; FGF-2; FGFB; fibroblast growth factor 2; fibroblast growth factor 2 (basic); HBGF-2; heparin-binding growth factor 2; prostatropin

Rabbit

IgG

Human

-20°C,2°C to 8°C,RT

12352203