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ProductsBack/PathPlus(tm) FOS / c-FOS Antibody (aa283-295)

PathPlus(tm) FOS / c-FOS Antibody (aa283-295)

LS-B14369
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityBovine, Canine, Equine, Guinea Pig, Human, Monkey, Porcine, Sheep
TargetFOS
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) FOS / c-FOS Antibody (aa283-295)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 37kD band observed in lysates of cell line HeLa (calculated MW of 40.7kD according to NP_005243.1). Recommended concentration: 0.3-1 ug/ml.. IHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.3 - 1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 37kD band observed in lysates of cell line HeLa (calculated MW of 40.7kD according to NP_005243.1). Recommended concentration: 0.3-1 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID2353
  • Target name
    FOS
  • Target description
    Fos proto-oncogene, AP-1 transcription factor subunit
  • Target synonyms
    AP-1, C-FOS, p55, protein c-Fos, FBJ murine osteosarcoma viral (v-fos) oncogene homolog (oncogene FOS), FBJ murine osteosarcoma viral oncogene homolog, Fos proto-oncogene, AP-1 trancription factor subunit, G0/G1 switch regulatory protein 7, activator protein 1, cellular oncogene c-fos, proto-oncogene c-Fos, transcription factor AP-1 subunit c-Fos
  • Host
    Goat
  • Reactivity
    Bovine, Canine, Equine, Guinea Pig, Human, Monkey, Porcine, Sheep
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of c-Fos/FOS using anti-c-Fos/FOS antibody (A00297-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human Jurkat whole cell lysates, Lane 3: human Sh-sy5y whole cell lysates, Lane 4: human A549 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-c-Fos/FOS antigen affinity purified polyclonal antibody (Catalog # A00297-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for c-Fos/FOS at approximately 50KD. The expected band size for c-Fos/FOS is at 50KD.
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