PathPlus(tm) GAP43 Antibody

LifeSpan BioSciences

PathPlus(tm) GAP43 Antibody

23

Recognizes rat GAP-43. Reacts with both phosphorylated and unphosphorylated forms of the GAP-43 protein.

Immunohistochemistry: LS-B2157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2157 was determined to be 5 ug/ml. Positive control: Cell lysates derived from mouse and rat brain.. ELISA (0.1 - 1 µg/ml), IHC, IHC-Fr (5 µg/ml), IHC-P (5 µg/ml), WB (1 µg/ml) Immunohistochemistry: LS-B2157 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B2157 was determined to be 5 ug/ml. Positive control: Cell lysates derived from mouse and rat brain.

Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin

Research Use Only

Monoclonal

0.5 mg/ml

Unconjugated

GAP43

growth associated protein 43

axonal membrane protein GAP-43; B-50; calmodulin-binding protein P-57; GAP-43; nerve growth-related peptide GAP43; neural phosphoprotein B-50; neuromodulin; neuron growth-associated protein 43; PP46; protein F1

Mouse

IgG2a

Chicken, Human, Mouse, Rabbit, Reptile, Rat

-20°C,2°C to 8°C

12352203