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ProductsBack/PathPlus(tm) NOS1 / nNOS Antibody (aa1457-1468)

PathPlus(tm) NOS1 / nNOS Antibody (aa1457-1468)

LS-B15177
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Equine, Human, Mammals, Monkey, Mouse, Rabbit
TargetNOS1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) NOS1 / nNOS Antibody (aa1457-1468)
  • Delivery Days Customer
    23
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (10 µg/ml), IHC-P (2.5 - 5 µg/ml), Peptide-ELISA (1:32000), WB (1 - 2 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID4842
  • Target name
    NOS1
  • Target description
    nitric oxide synthase 1
  • Target synonyms
    IHPS1, N-NOS, NC-NOS, NOS, bNOS, nNOS, nitric oxide synthase 1, NOS type I, constitutive NOS, neuronal NOS, nitric oxide synthase 1 (neuronal), peptidyl-cysteine S-nitrosylase NOS1
  • Host
    Goat
  • Reactivity
    Canine, Equine, Human, Mammals, Monkey, Mouse, Rabbit
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of nNOS (neuronal)/NOS1 using anti-nNOS (neuronal)/NOS1 antibody (A01070-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-nNOS (neuronal)/NOS1 antigen affinity purified polyclonal antibody (Catalog # A01070-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for nNOS (neuronal)/NOS1 at approximately 161 kDa. The expected band size for nNOS (neuronal)/NOS1 is at 161 kDa.
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