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ProductsBack/PathPlus(tm) PCNA Antibody (aa111-122)

PathPlus(tm) PCNA Antibody (aa111-122)

LS-B16699
LifeSpan BioSciences
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Porcine, Rat
TargetPCNA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) PCNA Antibody (aa111-122)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 30kD band observed in lysates of cell line Jurkat (calculated MW of 28.8kD according to NP_002583.1). Recommended concentration: 0.05-0.2 ug/ml.. IHC-P (2.5 µg/ml), Peptide-ELISA (1:128000), WB (0.03 - 0.1 µg/ml) Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 30kD band observed in lysates of cell line Jurkat (calculated MW of 28.8kD according to NP_002583.1). Recommended concentration: 0.05-0.2 ug/ml.
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5111
  • Target name
    PCNA
  • Target description
    proliferating cell nuclear antigen
  • Target synonyms
    ATLD2, proliferating cell nuclear antigen, DNA polymerase delta auxiliary protein, cyclin
  • Host
    Goat
  • Reactivity
    Human, Mouse, Porcine, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (A00125). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.
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