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ProductsBack/PathPlus(tm) PCNA Antibody (clone PC10)

PathPlus(tm) PCNA Antibody (clone PC10)

LS-B9987
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityCanine, Chicken, Equine, Feline, Fish, Ferret, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat, Sheep, Xenopus
TargetPCNA
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) PCNA Antibody (clone PC10)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo (1:100), IHC, IHC-Fr, IHC-P (10 µg/ml), IP, WB (1:1000)
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    PC10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5111
  • Target name
    PCNA
  • Target description
    proliferating cell nuclear antigen
  • Target synonyms
    ATLD2, proliferating cell nuclear antigen, DNA polymerase delta auxiliary protein, cyclin
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Canine, Chicken, Equine, Feline, Fish, Ferret, Hamster, Human, Monkey, Mouse, Primate, Rabbit, Rat, Sheep, Xenopus
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of PCNA using anti-PCNA antibody (A00125). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Hela whole cell lysates, Lane 2: human MCF-7 whole cell lysates, Lane 3: human 293T whole cell lysates, Lane 4: human HepG2 whole cell lysates, Lane 5: rat PC-12 whole cell lysates, Lane 6: mouse NIH/3T3 whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PCNA antigen affinity purified polyclonal antibody (Catalog # A00125) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PCNA at approximately 36 kDa. The expected band size for PCNA is at 29 kDa.
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