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PathPlus(tm) PEX19 Antibody

LS-B16426
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetPEX19
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) PEX19 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    The predicted MW is 29kDa/32kDa, while the observed MW by Western blot was 37kDa.
  • Applications
    ImmunoFluorescence, Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IF (1:50 - 1:200), IHC-P (1:100), WB (1:500 - 1:2000) The predicted MW is 29kDa/32kDa, while the observed MW by Western blot was 37kDa.
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID5824
  • Target name
    PEX19
  • Target description
    peroxisomal biogenesis factor 19
  • Target synonyms
    D1S2223E, HK33, PBD12A, PMP1, PMPI, PXF, PXMP1, peroxisomal biogenesis factor 19, 33 kDa housekeeping protein, housekeeping gene, 33kD, peroxin-19, peroxisomal farnesylated protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PEX19 using anti-PEX19 antibody (A03186-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human Jurkat whole cell lysates, Lane 2: human K562 whole cell lysates, Lane 3: human SiHa whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PEX19 antigen affinity purified polyclonal antibody (Catalog # A03186-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PEX19 at approximately 38 kDa. The expected band size for PEX19 is at 33 kDa.
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