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ProductsBack/PathPlus(tm) PLEKHG5 Antibody (clone 5A9)

PathPlus(tm) PLEKHG5 Antibody (clone 5A9)

LS-B16755
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetPLEKHG5
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) PLEKHG5 Antibody (clone 5A9)
  • Delivery Days Customer
    14
  • Application Supplier Note
    Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF, IHC-P (10 µg/ml), WB Immunohistochemistry: Formalin-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Recombinant protein.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5A9
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID57449
  • Target name
    PLEKHG5
  • Target description
    pleckstrin homology and RhoGEF domain containing G5
  • Target synonyms
    ARHGEF45, CMTRIC, DSMA4, GEF720, HMNR4, Syx, Tech, pleckstrin homology domain-containing family G member 5, NFkB activating protein, PH domain-containing family G member 5, guanine nucleotide exchange factor 720, novel PH domain-containing protein, pleckstrin homology domain containing, family G (with RhoGef domain) member 5, synectin-binding guanine exchange factor
  • Host
    Mouse
  • Isotype
    IgG2a
  • Reactivity
    Human
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PLEKHG5 using anti-PLEKHG5 antibody (A08522). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human SH-SY5Y whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLEKHG5 antigen affinity purified polyclonal antibody (Catalog # A08522) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PLEKHG5 at approximately 125 kDa. The expected band size for PLEKHG5 is at 111 kDa.
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