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PathPlus(tm) S100A4 / FSP1 Antibody

LS-B16162
LifeSpan BioSciences
ApplicationsImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetS100A4
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) S100A4 / FSP1 Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) Citrate buffer of pH 6.0 Recommended IHC controls tissues are Malignant melanoma. Resulting localization is Nuclear, Cytoplasmic
  • Applications
    ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:200 - 1:400), IHC-P (1:50) Use Heat-induced epitope retrieval (HIER) Citrate buffer of pH 6.0 Recommended IHC controls tissues are Malignant melanoma. Resulting localization is Nuclear, Cytoplasmic
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID6275
  • Target name
    S100A4
  • Target description
    S100 calcium binding protein A4
  • Target synonyms
    18A2, 42A, CAPL, FSP1, MTS1, P9KA, PEL98, protein S100-A4, S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog), calcium placental protein, fibroblast-specific protein-1, leukemia multidrug resistance associated protein, malignant transformation suppression 1, metastasin 1, murine placental homolog, placental calcium-binding protein, protein Mts1
  • Host
    Mouse
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

FAQ

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Figure 1. Western blot analysis of S100A4 using anti-S100A4 antibody (A01217-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human Hela whole cell lysates, Lane 3: human Hela whole cell lysates, Lane 4: human THP-1 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-S100A4 antigen affinity purified polyclonal antibody (Catalog # A01217-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for S100A4 at approximately 10-12KD. The expected band size for S100A4 is at 10-12KD.
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