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ProductsBack/PathPlus(tm) SOX9 Antibody (clone 3F11)

PathPlus(tm) SOX9 Antibody (clone 3F11)

LS-B4421
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetSOX9
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) SOX9 Antibody (clone 3F11)
  • Delivery Days Customer
    23
  • Application Supplier Note
    Western Blot (Cell lysate) - positive control HepG2. Immunofluorescence (10 ug/ml) - positive control HepG2 cells.
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    ELISA, IF (10 µg/ml), IHC, IHC-P (5 µg/ml), WB Western Blot (Cell lysate) - positive control HepG2. Immunofluorescence (10 ug/ml) - positive control HepG2 cells.
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    3F11
  • Concentration
    0.42 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6662
  • Target name
    SOX9
  • Target description
    SRY-box transcription factor 9
  • Target synonyms
    CMD1, CMPD1, ENH13, SRA1, SRXX2, SRXY10, TES, TESCO, transcription factor SOX-9, SRY (sex determining region Y)-box9, SRY (sex-determining region Y)-box 9 protein, SRY-box 9, SRY-related HMG-box, gene 9
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of SOX9 using anti-SOX9 antibody (A00177-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates Lane 2: human PC-3 whole cell lysates After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SOX9 antigen affinity purified polyclonal antibody (Catalog # A00177-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for SOX9 at approximately 70KD. The expected band size for SOX9 is at 56KD.
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