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ProductsBack/PathPlus(tm) SPP1 / Osteopontin Antibody

PathPlus(tm) SPP1 / Osteopontin Antibody

LS-B10122
LifeSpan BioSciences
ApplicationsImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman, Mouse, Rat
TargetSPP1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) SPP1 / Osteopontin Antibody
  • Delivery Days Customer
    23
  • Application Supplier Note
    SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 36 kDa Observed: 37 kDa. ELISA, IF (20 µg/ml), IHC, IHC-P (10 µg/ml), WB (1 - 2 µg/ml) SPP1 antibody can be used for detection of SPP1 by Western blot at 1 - 2 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 36 kDa Observed: 37 kDa
  • Applications
    ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID6696
  • Target name
    SPP1
  • Target description
    secreted phosphoprotein 1
  • Target synonyms
    BNSP, BSPI, ETA-1, OPN, osteopontin, SPP1/CALPHA1 fusion, early T-lymphocyte activation 1, lnc-PKD2-2-3, nephropontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), urinary stone protein, uropontin
  • Host
    Rabbit
  • Isotype
    IgG
  • Reactivity
    Human, Mouse, Rat
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of Osteopontin using anti-Osteopontin antibody (RP1080). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: human SHG-44 whole cell lysates, After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Osteopontin antigen affinity purified polyclonal antibody (Catalog # RP1080) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Osteopontin at approximately 66,25-55KD. The expected band size for Osteopontin is at 35KD.
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