PathPlus(tm) SPP1 / Osteopontin Antibody

LifeSpan BioSciences

PathPlus(tm) SPP1 / Osteopontin Antibody

23

Immunohistochemistry: LS-B425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B425 was determined to be 1:500.. ELISA (1:5000 - 1:20000), IHC, IHC-P (1:500), IP (1:100), WB (1:500 - 1:2000) Immunohistochemistry: LS-B425 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B425 was determined to be 1:500.

ImmunoPrecipitation, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

82 mg/ml

Unconjugated

...

SPP1

secreted phosphoprotein 1

BNSP, BSPI, ETA-1, OPN, osteopontin, SPP1/CALPHA1 fusion, early T-lymphocyte activation 1, lnc-PKD2-2-3, nephropontin, osteopontin/immunoglobulin alpha 1 heavy chain constant region fusion protein, secreted phosphoprotein 1 (osteopontin, bone sialoprotein I, early T-lymphocyte activation 1), urinary stone protein, uropontin

Rabbit

Canine, Human, Mouse, Porcine, Rat

-20°C,2°C to 8°C

12352203