PathPlus(tm) TGFB1 / TGF Beta 1 Antibody

LifeSpan BioSciences

PathPlus(tm) TGFB1 / TGF Beta 1 Antibody

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A BLAST analysis was used to suggest reactivity with this protein from most mammalian sources based on 100% homology for the immunogen sequence. Cross-reactivity is expected with TGFb1 from non-mammalian sources as only a single amino acid residue change

Immunohistochemistry: LS-B655 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B655 was determined to be 5 ug/ml.. ELISA (1:10000 - 1:20000), IF, IHC, IHC-P (5 µg/ml), WB (1:100 - 1:500) Immunohistochemistry: LS-B655 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B655 was determined to be 5 ug/ml.

ImmunoFluorescence, Western Blot, ELISA, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin

Research Use Only

Polyclonal

1.13 mg/ml

Unconjugated

...

TGFB1

transforming growth factor beta 1

CED; DPD1; IBDIMDE; LAP; latency-associated peptide; prepro-transforming growth factor beta-1; TGFB; TGFbeta; TGF-beta1; TGF-beta-1; transforming growth factor beta1; transforming growth factor beta-1 proprotein

Rabbit

IgG

Human

-20°C,2°C to 8°C

12352203