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ProductsBack/PathPlus(tm) THY1 / CD90 Antibody (clone 5E10, Azide-free)

PathPlus(tm) THY1 / CD90 Antibody (clone 5E10, Azide-free)

LS-B11449
LifeSpan BioSciences
ApplicationsFlow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityEquine, Human, Porcine, Primate
TargetTHY1
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) THY1 / CD90 Antibody (clone 5E10, Azide-free)
  • Delivery Days Customer
    23
  • Applications
    Flow Cytometry, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    Flo, ICC, IHC, IHC-Fr, IHC-P (20 µg/ml), IP, WB
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    5E10
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    >95%
  • Gene ID7070
  • Target name
    THY1
  • Target description
    Thy-1 cell surface antigen
  • Target synonyms
    CD90, CDw90, thy-1 membrane glycoprotein, Thy-1 T-cell antigen, thy-1 antigen
  • Host
    Mouse
  • Isotype
    IgG1
  • Reactivity
    Equine, Human, Porcine, Primate
  • Storage Instruction
    2°C to 8°C
  • UNSPSC
    41116161

FAQ

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Figure 1. Western blot analysis of CD90/Thy1 using anti-CD90/Thy1 antibody (A01818-1). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD90/Thy1 antigen affinity purified polyclonal antibody (Catalog # A01818-1) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for CD90/Thy1 at approximately 24 kDa. The expected band size for CD90/Thy1 is at 18 kDa.
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