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ProductsBack/PathPlus(tm) TOP2A / Topoisomerase II Alpha Antibody

PathPlus(tm) TOP2A / Topoisomerase II Alpha Antibody

LS-B16164
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
ReactivityHuman
TargetTOP2A
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) TOP2A / Topoisomerase II Alpha Antibody
  • Delivery Days Customer
    14
  • Application Supplier Note
    Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Tonsil, Spleen. Resulting localization is Cytoplasmic
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC (1:200 - 1:400), IHC-P (1:50), WB (1:200 - 1:400) Use Heat-induced epitope retrieval (HIER) TRIS-EDTA of pH 8.0 Recommended IHC controls tissues are Tonsil, Spleen. Resulting localization is Cytoplasmic
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Concentration
    1 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7153
  • Target name
    TOP2A
  • Target description
    DNA topoisomerase II alpha
  • Target synonyms
    TOP2, TOP2alpha, TOPIIA, TP2A, DNA topoisomerase 2-alpha, DNA gyrase, DNA topoisomerase (ATP-hydrolyzing), DNA topoisomerase II, 170 kD, DNA topoisomerase II, alpha isozyme, topoisomerase (DNA) II alpha 170kDa
  • Host
    Mouse
  • Isotype
    IgG
  • Reactivity
    Human
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of TOP2A using anti-TOP2A antibody (A00953). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human A549 whole cell lysates, Lane 2: human U2OS whole cell lysates, Lane 3: human HEK293 whole cell lysates, Lane 4: human K562 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOP2A antigen affinity purified polyclonal antibody (Catalog # A00953) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for TOP2A at approximately 174KD. The expected band size for TOP2A is at 174KD.
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