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ProductsBack/PathPlus(tm) TTR / Transthyretin Antibody

PathPlus(tm) TTR / Transthyretin Antibody

LS-A11189
LifeSpan BioSciences
ApplicationsWestern Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetTTR
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Overview

  • Supplier
    LifeSpan BioSciences
  • Product Name
    PathPlus(tm) TTR / Transthyretin Antibody
  • Delivery Days Customer
    14
  • Applications
    Western Blot, ImmunoHistoChemistry, ImmunoHistoChemistry Paraffin
  • Applications Supplier
    IHC-P (5 - 10 µg/ml), SWB (100 µg/ml)
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.88 mg/ml
  • Conjugate
    Unconjugated
  • Estimated Purity
    ...
  • Gene ID7276
  • Target name
    TTR
  • Target description
    transthyretin
  • Target synonyms
    ATTR, CTS, CTS1, HEL111, HsT2651, PALB, TBPA, TTN, transthyretin, epididymis luminal protein 111, prealbumin, amyloidosis type I, thyroxine-binding prealbumin
  • Host
    Rabbit
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of Prealbumin using anti-Prealbumin antibody (PB9749). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human placenta tissue lysate, Lane 2: human HCCP tissue lysate. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Prealbumin antigen affinity purified polyclonal antibody (Catalog # PB9749) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for Prealbumin at approximately 14 kDa. The expected band size for Prealbumin is at 14 kDa.
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