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PAX6 antibody

GTX113241
GeneTex
ApplicationsImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
Product group Antibodies
TargetPAX6
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Overview

  • Supplier
    GeneTex
  • Product Name
    PAX6 antibody
  • Delivery Days Customer
    9
  • Application Supplier Note
    WB: 1:500-1:3000. ICC/IF: 1:100-1:1000. IHC-P: 1:100-1:1000. IHC-Fr: 1:100-1:1000. IP: 1:1000-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
  • Applications
    ImmunoFluorescence, ImmunoPrecipitation, Western Blot, ImmunoCytoChemistry, ImmunoHistoChemistry, ImmunoHistoChemistry Frozen, ImmunoHistoChemistry Paraffin
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.7 mg/ml
  • Conjugate
    Unconjugated
  • Gene ID5080
  • Target name
    PAX6
  • Target description
    paired box 6
  • Target synonyms
    AN; AN1; AN2; Aniridia 1; Aniridia 2; aniridia type II protein; ASGD5; D11S812E; FVH1; MGDA; oculorhombin; paired box homeotic gene-6; paired box protein Pax-6; WAGR
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP26367
  • Protein Name
    Paired box protein Pax-6
  • Scientific Description
    This gene encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peters anomaly. Alternatively spliced transcript variants encoding either the same or different isoform have been found for this gene. [provided by RefSeq]
  • Storage Instruction
    -20°C or -80°C,2°C to 8°C
  • UNSPSC
    12352203

References

  • Zika virus-induces metabolic alterations in fetal neuronal progenitors that could influence in neurodevelopment during early pregnancy.
    Read more
  • Glycolysis mediates neuron specific histone acetylation in valproic acid-induced human excitatory neuron differentiation.
    Read more
  • Differential Expression Levels of Sox9 in Early Neocortical Radial Glial Cells Regulate the Decision between Stem Cell Maintenance and Differentiation. Fabra-Beser J et al., 2021 Aug 18, J Neurosci
    Read more
  • BASP1 labels neural stem cells in the neurogenic niches of mammalian brain. Manganas LN et al., 2021 Mar 10, Sci Rep
    Read more
  • Down-regulation of ATF1 leads to early neuroectoderm differentiation of human embryonic stem cells by increasing the expression level of SOX2. Yang SC et al., 2019 Sep, FASEB J
    Read more
  • OLIG2 Drives Abnormal Neurodevelopmental Phenotypes in Human iPSC-Based Organoid and Chimeric Mouse Models of Down Syndrome. Xu R et al., 2019 Jun 6, Cell Stem Cell
    Read more
  • Neural Progenitor-Like Cells Induced from Human Gingiva-Derived Mesenchymal Stem Cells Regulate Myelination of Schwann Cells in Rat Sciatic Nerve Regeneration. Zhang Q et al., 2017 Feb, Stem Cells Transl Med
    Read more
  • Role of astroglia in Downs syndrome revealed by patient-derived human-induced pluripotent stem cells. Chen C et al., 2014 Jul 18, Nat Commun
    Read more
  • SMN is required for the maintenance of embryonic stem cells and neuronal differentiation in mice. Chang WF et al., 2015, Brain Struct Funct
    Read more
  • MicroRNA-328 may influence myopia development by mediating the PAX6 gene. Chen KC et al., 2012 May 31, Invest Ophthalmol Vis Sci
    Read more

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Figure 1. Western blot analysis of PAX6 using anti-PAX6 antibody (PB9768). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX6 antigen affinity purified polyclonal antibody (Catalog # PB9768) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX6 at approximately 47 kDa. The expected band size for PAX6 is at 47 kDa.
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WB analysis of various sample lysates using GTX55739 PAX6 antibody. The signal was developed with ECL plus-Enhanced. Dilution : 1:1000 Loading : 25microg per lane
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