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Anti-PAX6 [RAB-T42]

Ab01831-1.1
Absolute Antibody
ApplicationsFlow Cytometry, ImmunoFluorescence
Product group Antibodies
TargetPAX6
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Overview

  • Supplier
    Absolute Antibody
  • Product Name
    Anti-PAX6 [RAB-T42]
  • Delivery Days Customer
    7
  • Application Supplier Note
    This antibody has been used and recommended for the following methods: FC; spiked-IP; IF.
  • Applications
    Flow Cytometry, ImmunoFluorescence
  • Applications Supplier
    FC; spiked-IP; IF
  • Certification
    Research Use Only
  • Clonality
    Monoclonal
  • Clone ID
    RAB-T42
  • Gene ID5080
  • Target name
    PAX6
  • Target description
    paired box 6
  • Target synonyms
    AN; AN1; AN2; Aniridia 1; Aniridia 2; aniridia type II protein; ASGD5; D11S812E; FVH1; MGDA; oculorhombin; paired box homeotic gene-6; paired box protein Pax-6; WAGR
  • Host
    Mouse
  • Isotype
    IgG1
  • Protein IDP26367
  • Protein Name
    Paired box protein Pax-6
  • Scientific Description
    This chimeric mouse antibody was made using the variable domain sequences of the original Human Fab format, for improved compatibility with existing reagents, assays and techniques.
  • Reactivity Supplier
    Human
  • Reactivity Supplier Note
    This antibody was obtained by recombinant antibody (rAb) phage display recognizing PAX6 protein under non-denaturing conditions.
  • Storage Instruction
    -20°C,2°C to 8°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAX6 using anti-PAX6 antibody (PB9768). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX6 antigen affinity purified polyclonal antibody (Catalog # PB9768) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX6 at approximately 47 kDa. The expected band size for PAX6 is at 47 kDa.
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