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ProductsBack/Goat anti-PAX6 (internal)

Goat anti-PAX6 (internal)

EB07772
Everest Biotech
ApplicationsFlow Cytometry, Western Blot, ELISA
Product group Antibodies
TargetPAX6
100 ug
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Overview

  • Supplier
    Everest Biotech
  • Product Name
    Goat anti-PAX6 (internal) Antibody
  • Delivery Days Customer
    5
  • Application Supplier Note
    Flow Cytometry: Flow cytometric analysis of U251 cells. Recommended concentration: 10ug/ml.
  • Applications
    Flow Cytometry, Western Blot, ELISA
  • Applications Supplier
    Pep-ELISA, WB, FC
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Concentration
    0.5 mg/ml
  • Gene ID5080
  • Target name
    PAX6
  • Target description
    paired box 6
  • Target synonyms
    AN; AN1; AN2; Aniridia 1; Aniridia 2; aniridia type II protein; ASGD5; D11S812E; FVH1; MGDA; oculorhombin; paired box homeotic gene-6; paired box protein Pax-6; WAGR
  • Host
    Goat
  • Scientific Description
    RefSeq number(s): NP_000271.1; NP_001595.2; NP_001297088.1; NP_001355839.1; NP_001355840.1; NP_001355841.1; NP_001355844.1; NP_001355847.1; NP_001355849.1; NP_001355850.1; NP_001355851.1; NP_001355857.1; NP_001355858.1; NP_001355859.1. GeneIDs all Nonhuman: 18508 (mouse); 25509 (rat);. Purification: Antigen affinity purified. Names and symbols: PAX6; paired box gene 6 (aniridia, keratitis); AN; AN2; D11S812E; MGC17209; MGDA; WAGR; paired box gene 6; paired box homeotic gene 6 (aniridia, keratitis); paired box homeotic gene-6
  • Reactivity Supplier
    Human, Mouse, Rat, Fog, Cow
  • Storage Instruction
    -20°C
  • UNSPSC
    12352203

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Figure 1. Western blot analysis of PAX6 using anti-PAX6 antibody (PB9768). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Lane 1: Rat Brain Tissue Lysate at 50ug, Lane 2: Mouse Brain Tissue Lysate at 50ug, Lane 3: U87 Whole Cell Lysate at 40ug, Lane 4: HELA Whole Cell Lysate at 40ug. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAX6 antigen affinity purified polyclonal antibody (Catalog # PB9768) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PAX6 at approximately 47 kDa. The expected band size for PAX6 is at 47 kDa.
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