Western blot All lanes: PBLD antibody at 6microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa

PBLD Antibody

CSB-PA017501LA01HU

ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry

Product group Antibodies

ReactivityHuman, Mouse

TargetPBLD

Overview

Supplier
Cusabio
Product Name
PBLD Antibody
Delivery Days Customer
20
Applications
Western Blot, ELISA, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID64081
Target name
PBLD
Target description
phenazine biosynthesis like protein domain containing
Target synonyms
epididymis secretory protein Li 306; epididymis secretory sperm binding protein; HEL-S-306; MAWBP; MAWD-binding protein; MAWDBP; phenazine biosynthesis-like domain-containing protein
Host
Rabbit
Isotype
IgG
Protein IDP30039
Protein Name
Phenazine biosynthesis-like domain-containing protein
Scientific Description
cytoplasm, extracellular exosome, maintenance of gastrointestinal epithelium, negative regulation of epithelial cell migration, negative regulation of epithelial cell proliferation, negative regulation of epithelial to mesenchymal transition, negative regulation of pathway-restricted SMAD protein phosphorylation, negative regulation of SMAD protein import into nucleus, negative regulation of transforming growth factor beta receptor signaling pathway
Reactivity
Human, Mouse
Storage Instruction
-20°C or -80°C
UNSPSC
41116161

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Figure 1. Western blot analysis of PBLD using anti-PBLD antibody (A10149-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBLD antigen affinity purified polyclonal antibody (Catalog # A10149-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBLD at approximately 32 kDa. The expected band size for PBLD is at 32 kDa.

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$Antigen: bs-9032P, 2ug/100ul \nPrimary: Antiserum, 1:500, 1:1000, 1:2000, 1:4000, 1:8000, 1:16000, 1:32000; \nSecondary: HRP conjugated Rabbit Anti-Goat IgG(bs-0294R-HRP) at 1: 5000; \nTMB staining\nRead the data in Microplate Reader by 450nm. \n$