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Western blot All lanes: PBLD antibody at 6microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa
Western blot All lanes: PBLD antibody at 6microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa
Western blot All lanes: PBLD antibody at 6microg/ml Lane 1: Mouse liver tissue Lane 2: Mouse kidney tissue Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 32 kDa Observed band size: 32 kDa

PBLD Antibody

CSB-PA017501LA01HU
Cusabio
ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry
Product group Antibodies
ReactivityHuman, Mouse
TargetPBLD
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Overview

  • Supplier
    Cusabio
  • Product Name
    PBLD Antibody
  • Delivery Days Customer
    20
  • Applications
    Western Blot, ELISA, ImmunoHistoChemistry
  • Certification
    Research Use Only
  • Clonality
    Polyclonal
  • Conjugate
    Unconjugated
  • Gene ID64081
  • Target name
    PBLD
  • Target description
    phenazine biosynthesis like protein domain containing
  • Target synonyms
    HEL-S-306, MAWBP, MAWDBP, phenazine biosynthesis-like domain-containing protein, MAWD-binding protein, epididymis secretory protein Li 306, epididymis secretory sperm binding protein
  • Host
    Rabbit
  • Isotype
    IgG
  • Protein IDP30039
  • Protein Name
    Phenazine biosynthesis-like domain-containing protein
  • Scientific Description
    cytoplasm, extracellular exosome, maintenance of gastrointestinal epithelium, negative regulation of epithelial cell migration, negative regulation of epithelial cell proliferation, negative regulation of epithelial to mesenchymal transition, negative regulation of pathway-restricted SMAD protein phosphorylation, negative regulation of SMAD protein import into nucleus, negative regulation of transforming growth factor beta receptor signaling pathway
  • Reactivity
    Human, Mouse
  • Storage Instruction
    -20°C or -80°C
  • UNSPSC
    41116161

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Figure 1. Western blot analysis of PBLD using anti-PBLD antibody (A10149-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBLD antigen affinity purified polyclonal antibody (Catalog # A10149-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBLD at approximately 32 kDa. The expected band size for PBLD is at 32 kDa.
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