ProductsBack/PBLD Polyclonal Antibody

PBLD Polyclonal Antibody

CAC13857

ApplicationsWestern Blot, ELISA, ImmunoHistoChemistry

Product group Antibodies

TargetPBLD

Overview

Supplier
Biomatik
Product Name
PBLD Polyclonal Antibody
Delivery Days Customer
12
Applications
Western Blot, ELISA, ImmunoHistoChemistry
Certification
Research Use Only
Clonality
Polyclonal
Conjugate
Unconjugated
Gene ID64081
Target name
PBLD
Target description
phenazine biosynthesis like protein domain containing
Target synonyms
epididymis secretory protein Li 306; epididymis secretory sperm binding protein; HEL-S-306; MAWBP; MAWD-binding protein; MAWDBP; phenazine biosynthesis-like domain-containing protein
Host
Rabbit
Isotype
IgG
Protein IDP30039
Protein Name
Phenazine biosynthesis-like domain-containing protein
Scientific Description
The PBLD Polyclonal Antibody (Species: Human) has been validated for the following applications: ELISA, WB, IHC.
Storage Instruction
-20°C,2°C to 8°C
UNSPSC
12352203

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Figure 1. Western blot analysis of PBLD using anti-PBLD antibody (A10149-2). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: human hepatocellular carcinoma tumor tissue (HCCT) lysates, Lane 2: human hepatocellular carcinoma paracancerous tissue (HCCP) lysates. Lane 3: rat liver tissue lysates, Lane 4: rat RH35 whole cell lysates, Lane 5: mouse liver tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PBLD antigen affinity purified polyclonal antibody (Catalog # A10149-2) at 0.5 microg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002) with Tanon 5200 system. A specific band was detected for PBLD at approximately 32 kDa. The expected band size for PBLD is at 32 kDa.

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