HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PBX1 antibody [N2C3] (GTX105260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
![PBX1 antibody [N2C3] detects PBX1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PBX1 protein stained by PBX1 antibody [N2C3] (GTX105260) diluted at 1:500. Blue: Hoechst 33342 staining.](https://www.genetex.com/upload/website/prouct_img/normal/GTX105260/GTX105260_39974_IFA_w_23060120_912.webp "PBX1 antibody [N2C3] detects PBX1 protein at nucleus and cytoplasm by immunofluorescent analysis. Sample: SK-N-AS cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PBX1 protein stained by PBX1 antibody [N2C3] (GTX105260) diluted at 1:500. Blue: Hoechst 33342 staining.")
HeLa whole cell and nuclear extracts (30 μg) were separated by 10% SDS-PAGE, and the membrane was blotted with PBX1 antibody [N2C3] (GTX105260) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
PBX1 antibody [N2C3]
GTX105260
ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
Product group Antibodies
ReactivityHuman
TargetPBX1
Overview
- SupplierGeneTex
- Product NamePBX1 antibody [N2C3]
- Delivery Days Customer9
- Application Supplier NoteWB: 1:500-1:3000. ICC/IF: 1:100-1:1000. *Optimal dilutions/concentrations should be determined by the researcher.Not tested in other applications.
- ApplicationsImmunoFluorescence, Western Blot, ImmunoCytoChemistry
- CertificationResearch Use Only
- ClonalityPolyclonal
- Concentration1 mg/ml
- ConjugateUnconjugated
- Gene ID5087
- Target namePBX1
- Target descriptionPBX homeobox 1
- Target synonymsCAKUHED, pre-B-cell leukemia transcription factor 1, homeobox protein PBX1, homeobox protein PRL, pre-B-cell leukemia homeobox 1
- HostRabbit
- IsotypeIgG
- Protein IDP40424
- Protein NamePre-B-cell leukemia transcription factor 1
- ReactivityHuman
- Storage Instruction-20°C or -80°C,2°C to 8°C
- UNSPSC12352203
Datasheet
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